[buster-discuss] rhofit ??

[Jonathan Grimes]

   thanks.......just wanted to check...
     j.


Dr. Jonathan M. Grimes, 
NDM Senior Reseach Fellow
University Research Lecturer
Division of Structural Biology
Wellcome Trust Centre for Human Genetics
University of Oxford
Roosevelt Drive,
Oxford OX3 7BN, UK

Email: Jonathan at strubi.ox.ac.uk, Web: www.strubi.ox.ac.uk 
Tel: (+44) - 1865 - 287561, FAX: (+44) - 1865 - 287547       



On 29 Jun 2011, at 18:08, Oliver Smart wrote:

> On Wed, 29 Jun 2011, Jonathan Grimes wrote:
> 
>> 
>>  Dear Buster Developers,
>> 
>>   We have solved the structure of a viral polymerase bound
>>   to viral adaptor protein.   We can see clear density for a peptide
>>   10-15aa, so much smaller than the full length adaptor
>>   protein.   We dont know if the rest of the adaptor protein is in crystal (and
>>   is disordered) or had been cleaved.
>>   We are also unclear of the peptide sequence at the moment (data to ~2.3A).
>> 
>>   Is ligand fitting Rhofit appropriate to use ??  Ive never used Rhofit but have
>>  only heard good stories.
>> 
>>   Thanks
>>   Jon
>> 
> 
> 
> Jon,
> 
> We have never tried rhofit on something this big. It handles cyclodextran but this sound like a case for normal coot type building?
> 
> regards,
> 
> Oliver
> 
> | Dr Oliver Smart                             |
> | Global Phasing Ltd., Cambridge UK           |
> | http://www.globalphasing.com/people/osmart/ |
>