[buster-discuss] Coot not displaying disulfide bonds after buster refinement

[Donald Damian Raymond]

Hi all,

I've been having this issues for some time now and cannot seem to figure it out. Whenever, I refine my structure in buster and visualize the result in COOT, the disulfide bonds are not displayed. However, if I refine the structure in refmac or phenix, the S--S show up just fine in COOT.

Buster records for SSBOND below

SSBOND   1 CYS A   43    CYS A  276                          1555   1555  2.04
SSBOND   2 CYS A   56    CYS A   68                          1555   1555  2.03
SSBOND   3 CYS A   91    CYS A  137                          1555   1555  2.02
SSBOND   4 CYS A  280    CYS A  304                          1555   1555  2.04
SSBOND   5 CYS B   43    CYS B  276                          1555   1555  2.04
SSBOND   6 CYS B   56    CYS B   68                          1555   1555  2.03
SSBOND   7 CYS B   91    CYS B  137                          1555   1555  2.05
SSBOND   8 CYS B  280    CYS B  304                          1555   1555  2.05
SSBOND   9 CYS C   22    CYS C   96                          1555   1555  2.04
SSBOND  10 CYS C  152    CYS C  208                          1555   1555  2.04
SSBOND  11 CYS D   23    CYS D   89                          1555   1555  2.05
SSBOND  12 CYS D  135    CYS D  195                          1555   1555  2.04
SSBOND  13 CYS H   22    CYS H   96                          1555   1555  2.04
SSBOND  14 CYS H  152    CYS H  208                          1555   1555  2.03
SSBOND  15 CYS L   23    CYS L   89                          1555   1555  2.04
SSBOND  16 CYS L  135    CYS L  195                          1555   1555  2.04

Refmac records for SSBONDS below

SSBOND   1 CYS A   43    CYS A  276                                             
SSBOND   2 CYS A   56    CYS A   68                                             
SSBOND   3 CYS A   91    CYS A  137                                             
SSBOND   4 CYS A  137    CYS A   91                                             
SSBOND   5 CYS A  276    CYS A   43                                             
SSBOND   6 CYS A  280    CYS A  304                                             
SSBOND   7 CYS B   43    CYS B  276                                             
SSBOND   8 CYS B   56    CYS B   68                                             
SSBOND   9 CYS B   91    CYS B  137                                             
SSBOND  10 CYS B  137    CYS B   91                                             
SSBOND  11 CYS B  276    CYS B   43                                             
SSBOND  12 CYS B  280    CYS B  304                                             
SSBOND  13 CYS C   22    CYS C   96                                             
SSBOND  14 CYS C  152    CYS C  208                                             
SSBOND  15 CYS D   23    CYS D   89                                             
SSBOND  16 CYS D  135    CYS D  195     

Any help in this matter would be greatly appreciated.

Best,
Donald

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