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<p class="MsoNormal"><span style="font-size:11.0pt">Hello all,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">I'm refining a structure at ~1.8 A with 4 copies of a protein molecule in the AU. This was co-crystallized and also soaked with a large amount of peptide in the cryo (unknown binding site).<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">After seeing nothing in Fo-Fc maps from Refmac and Phenix I tried running autoBUSTER for the first time. Refinement using NCS and TLS gave R-20 R-free 22. More importantly I can now see some weak density for
1 copy of my peptide in the Fo-Fc map. <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">I have tried to build the peptide in coot but I am not 100% sure in the direction.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Does anyone have any advice on how to improve the maps further?<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Best <o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt">Trevor<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt"><o:p> </o:p></span></p>
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