<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div class=""><br class=""></div><div class=""> we have a low res data set, with 80% solvent</div><div class=""> and 3.5 dimers in the asym unit. the dimer is a pair of </div><div class=""> alpha helices (the molecule is a bZIP protein). There is </div><div class=""> disulphide that stabilises the dimer, which we also see on </div><div class=""> gels.</div><div class=""><br class=""></div><div class=""> we have been asked during the review process to delete the </div><div class=""> cysteines (ive changed to glycine) and rerefine and calc omit </div><div class=""> maps ….ie to show big blobs where disulphide is.</div><div class=""><br class=""></div><div class=""> is there a way of restraining the helices to be helices…..what happens </div><div class=""> is the carbonyls of the glycine refine into the sidechain density</div><div class=""><br class=""></div><div class=""> thanks</div><div class=""> jon</div><div class=""><br class=""></div><div class=""><br class=""></div><br class=""><div class="">
<div dir="auto" style="caret-color: rgb(0, 0, 0); color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-variant-east-asian: normal; font-variant-position: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Prof. Jonathan M. Grimes, <br class="">NDM Senior Research Fellow<br class="">DIAMOND Research Fellow<br class=""><br class="">Division of Structural Biology<br class="">Wellcome Centre for Human Genetics<br class="">University of Oxford<br class="">Roosevelt Drive,<br class="">Oxford OX3 7BN, UK<br class=""><br class=""><a href="mailto:Jonathan@strubi.ox.ac.uk" class="">Email: Jonathan@strubi.ox.ac.uk</a>, Web: <a href="http://www.strubi.ox.ac.uk" class="">www.strubi.ox.ac.uk</a> <br class="">Tel: (+44) - 1865 - 287561, FAX: (+44) - 1865 - 287547 </div></div></div></div></div>
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