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<p class="MsoNormal">Dear BUSTER users,<br>
<br>
Following the recent BUSTER release (20210716) we have received many useful<br>
questions about restraint generation with the new Grade2 tool. As similar<br>
questions often arise we have produced an FAQs page for Grade2:<br>
<br>
<a href="https://gphl.gitlab.io/grade2_docs/faqs.html">https://gphl.gitlab.io/grade2_docs/faqs.html</a><br>
<br>
Currently, this has answers to the questions:<br>
<br>
1. How can I check Grade2 is correctly installed and works?<br>
2. I have a problem with Grade2: what should I do?<br>
3. How to cite Grade2?<br>
4. How can I use Grade2 to generate a restraint dictionary with atom names<br>
consistent with an existing Grade dictionary?<br>
5. How can I run Grade2 if I only have a PDB file for the ligand?<br>
6. How can I produce restraints for a ligand with a different protonation<br>
state or tautomer?<br>
7. What are the Grade2/BUSTER restrictions on residue name?<br>
<br>
There is also a page for known issues:<br>
<br>
<a href="https://gphl.gitlab.io/grade2_docs/issues.html">https://gphl.gitlab.io/grade2_docs/issues.html</a><br>
<br>
The most important known issue with the first release is that<br>
"The --big_planes option produces messed-up restraints".<br>
<br>
Thanks to everyone for asking questions please keep them coming.<br>
<br>
As always, we kindly ask that support requests and feedback be sent to our<br>
developer mailing lists:<br>
<br>
buster-develop at globalphasing.com for BUSTER, Pipedream, Rhofit, Grade and Grade2<br>
<br>
rather than to individual developers.<br>
<br>
Oliver and the BUSTER developers<br>
<br>
<o:p></o:p></p>
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