[sharp-discuss] One question about sharp and the other phasing mathods

Clemens Vonrhein vonrhein@globalphasing.com
Mon, 12 May 2003 10:23:07 +0100


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Dear Zhiyong,

just go to

  http://www.globalphasing.com/mailman/listinfo/sharp-discuss

where you can subscribe, browse the archives ...

Cheers

Clemens

On Mon, May 12, 2003 at 05:19:08PM +0800, Lou Zhiyong wrote:
> Dear Clemens,
> I have usually send my message to discussion group from the control
> panel, and could you tell me how to "subscribe to this discussion list"?
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> Thank you very much!
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> Cheers,
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> Zhiyong
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> > Dear Zhiyong,
> >=20
> > first: could you please subscribe to this discussion list before
> > sending messages? Otherwise they are not getting through immediately
> > (and I have to approve/reject them by hand each time).
> >=20
> > Regarding differences: the best thing is to read the various papers
> > about the programs. For SHARP this would be
> >=20
> >     La Fortelle, E. de & Bricogne, G. (1997) Methods in
> >     Enzymology, Macromolecular Crystallography, volume
> >     276, pp. 472-494, edited by R. M. Sweet and C. W.
> >     Carter, Jr. New York: Academic Press.
> >=20
> > Regarding performance/results: did you use SHARP or autoSHARP? What
> > kind of experiment (MAD, SAD, SIR(AS), MAD+native, ...) did you do?
> > What were the messages/warnings that autoSHARP gave? How did the
> > refinement in SHARP behave? Have you tried both hands? ...
> >=20
> >=20
> > The current method for finding heavy atom sites in autoSHARP is
> > probably less powerful than using e.g. SHELXD (or SnB or Solve or
> > ...). So if the sites found by autoSHARP are not very good (or just
> > plain wrong): you can easily use any set of sites found by another
> > program and input these (in fractional coordinates) through the use of
> > a *.hatom file: see
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> >   http://www.globalphasing.com/sharp/manual/autoSHARP1.html#datafiles
> >=20
> > (in the next release you can use e.g. SHELXD PDB files directly).
> >=20
> > Without more information about your data and the exact runs you've
> > done it is very difficult to tell exactly where you could improve
> > things in SHARP/autoSHARP.
> >=20
> > Hope that helps (a bit).
> >=20
> > Cheers
> >=20
> > Clemens
> >=20
> > On Mon, May 12, 2003 at 04:37:13PM +0800, Lou Zhiyong wrote:
> > > Dear Sir,
> > > I am a fresh user of SHARP. After I have finished some work in SHARP,=
 I=20
> > > found the result of one data set(50-2.8) which gotten in SHARP is not=
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> > > good as which gotten in solve/resolve.
> > > But some others told me that SHARP is more powerful than solve/resolv=
e.
> > >=20
> > > Could you do me a favor to tell me the difference of SHARP, SOLVE,=20
> > > SHELX, and the other phasing mathods which having been used now. In=
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> > > general, what is the resolution range of data of each mathod, and the=
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> > > evaluation of each mathod.
> > >=20
> > > Thank you very much!
> > >=20
> > > Best Regards,
> > > Zhiyong Lou
> > >=20
> > >=20
> > > _______________________________________________
> > > sharp-discuss mailing list
> > > sharp-discuss@globalphasing.com
> > > http://www.globalphasing.com/mailman/listinfo/sharp-discuss
> >=20
> > --=20
> >=20
> > ***************************************************************
> > * Clemens Vonrhein, Ph.D.     vonrhein AT GlobalPhasing DOT com
> > *
> > *  Global Phasing Ltd.
> > *  Sheraton House, Castle Park     Tel: +44-(0)1223-353033
> > *  Cambridge CB3 0AX, UK           Fax: +44-(0)1223-366889
> > *--------------------------------------------------------------
> > * BUSTER Development Group      (http://www.globalphasing.com)
> > ***************************************************************
>=20

--=20

***************************************************************
* Clemens Vonrhein, Ph.D.     vonrhein AT GlobalPhasing DOT com
*
*  Global Phasing Ltd.
*  Sheraton House, Castle Park     Tel: +44-(0)1223-353033
*  Cambridge CB3 0AX, UK           Fax: +44-(0)1223-366889
*--------------------------------------------------------------
* BUSTER Development Group      (http://www.globalphasing.com)
***************************************************************

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