[sharp-discuss] FFT problem during solvent flattening

Clemens Vonrhein vonrhein at globalphasing.com
Mon Apr 11 14:19:22 BST 2005


Dear Beatriz,
 
it seems as if it isn't able to find a MTZ file ... weird. Especially
since it is a FFT step somewhere inside the density modification
loops.

One possible reason could be problems with your NFS setup. If your
sharpfiles/logfiles* sirectory/directories are on a NFS mounted
filesystem, make sure that the NFS fileserver and your local machine
have the same time (maybe one has daylight savings time and the other
hasn't). Since a lot of file writing and reading goes on inside the
density modification procedure, any problem with time synching will
give problems (a program like FFT wants to read a file that the NFS
server hasn't confirmed as beeing written yet).

Also, if one machine is a Linux box and another is a SGI you might get
into trouble (there are issues in the NFS interpretation on SGI that
are problematic).

This might not be very helpful, but it looks somethning more related
to your system setup and not inside SHARP/autoSHARP (but I might be
wrong).

Cheers

Clemens



On Wed, Apr 06, 2005 at 07:49:56AM -0300, Beatriz Guimaraes wrote:
> Dear all,
> 
> I am trying to run a solvent flattening protocol through the Phase 
> Improvement Control Panel but I get the error message bellow. FTT runs 
> fine from a unix shell or ccp4i.
> Any suggestions are appreciated!
> 
> Beatriz
> 
> 
>  ###############################################################
>  ### CCP4 5.0: FFT                version 5.0       : 26/01/04##
>  ###############################################################
>  User: sharp  Run date:  5/ 4/2005 Run time: 10:10:23
> 
>  Please reference: Collaborative Computational Project, Number 4. 1994.
>  "The CCP4 Suite: Programs for Protein Crystallography". Acta Cryst. 
> D50, 760-763.
>  as well as any specific reference in the program write-up.
> 
>  Data line--- TITL 100.000-1.90 A, after 1 density modification cycles
>  Data line--- LABI F1=Fmodori PHI=PHImodori
>  Data line--- RESO 100.000 1.90
>  Data line--- GRID 312 64 120
>  Data line--- XYZL ASU
>  Map limits will be set to the CCP4 asymmetric unit
>  Data line--- END
> 
> >>>>>>>>>>>> CCP4 library signal library_file:End of File (Error)
> 
> 	 raised in ccp4_file_raw_read <<<<<<
> 
> >>>>>>>>>>>> System signal 2:No such file or directory (Error)
> 
> 	 raised in ccp4_file_readchar <<<<<<
> 
> >>>>>>>>>>>> CCP4 library signal mtz:Read failed (Error)
> 
> 	 raised in MtzGet <<<<<<
> Error: failed to open file for read!
>  *** NO SUCH FILE !**
>  FFT:   ** File does not exist **
>  FFT:   ** File does not exist **
> 
> 
> 
> 
> 
> -- 
> Beatriz Gomes Guimarães
> Laboratório Nacional de Luz Síncrotron
> Centro de Biologia Molecular Estrutural
> Caixa Postal 6192  CEP 13084-971
> Campinas SP  Brasil
> Tel: +55-19-35121106   Fax: +55-19-35121006
> E-mail: beatriz at lnls.br
> 
> 
> _______________________________________________
> sharp-discuss mailing list
> sharp-discuss at globalphasing.com
> http://www.globalphasing.com/mailman/listinfo/sharp-discuss
> 

-- 

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* Clemens Vonrhein, Ph.D.     vonrhein AT GlobalPhasing DOT com
*
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