[sharp-discuss] HLAshasol, patched SIGMAA

[Clemens Vonrhein]

Dear Imre,

for buccaneer the HLAdm-HLDdm should be fine. I guess it only uses the
HLs to calculate the initial map?

I usually look at the SOLOMON maps, since I find them 'nicer'
(whatever that means) than the DM maps. Probably because the masking
in SOLOMON (based on local-standard-deviation in a small sphere) gives
a bit more detail than the method in DM (auto-WANG mask: usually
rather large spheres). But this is personal choice I guess. So you
could also convert the FBshasol/PHIBshasol into HLs: there is a
utility coming with CCP4/Clipper.

Cheers

Clemens


On Thu, Apr 26, 2007 at 03:05:15PM +0200, Imre Toeroe wrote:
> Dear Clemens,
> 
> The reason I needed them was to go to Cowtan's buccaneer directly from
> solomon as it requires HLs. I'll try your suggestion, thanks.
> 
> Imre
> 
> Clemens Vonrhein wrote:
> > Dear Imre,
> >
> > this was a patch to SIGMAA a couple of CCP4 releases ago. I wouldn't
> > recommend it nowadays (will need to go back and re-implement the patch
> > to the latest CCP4 sigmaa version).
> >
> > You should also have HLAdm, HLBdm, HLCdm and HLDdm in your
> > eden_flat_*pc.mtz file: these are the Hendrickson-Lattman after the
> > final DM run (which is done after the SOLOMON solvent
> > flipping/flattening stage). You should be fine in using those.
> >
> > Cheers
> >
> > Clemens
> >
> > On Thu, Apr 26, 2007 at 07:51:07AM +0200, Imre Toeroe wrote:
> >   
> >> Dear SHARPers,
> >>
> >> I have found this in the documentation concerning the mtz file produced
> >> after solvent flattening:
> >>
> >> "HLAshasol, HLBshasol, HLCshasol, HLDshasolHendrickson-Lattman
> >> coefficientsdescription of phase probability (after density modification
> >> with SOLOMON - only available if a patched SIGMAA version is used)"
> >>
> >>
> >> Does anybody of you know where can we obtain the patch against SIGMAA to
> >> produce HL[ABCD}shasol too?
> >>
> >> Thanks,
> >>
> >> Imre
> >>
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> >>
> >>     
> >
> >   
> 
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