[sharp-discuss] ISYM column

[Phil Evans]

ctruncate ought to output ISYM and may do in future when someone gets round to it.
ctruncate gives some more useful statistics than old truncate.
In due course I shall probably incorporate ctruncate into my successor program to Scala (working title Aimless), and I'll probably output ISYM (but see point 2  below)

The longer answers are:-

1. ISYM only makes a marginal difference anyway in nearly all cases (though it should be used) 

2. there has been a (slow) move in the CCP4 programs to use F+ & F- (or indeed I+ & I-), rather than Fmean & Dano, which is arguably better, since programs can then decide themselves what to do

3. Sharp/autoSharp works on files without ISYM as long as you give a blank assignment

Phil

On 24 Aug 2010, at 23:06, Florian Schmitzberger wrote:

> Dear David,
> 
> Thank you! Done.
> 
> Out of curiosity, does anybody know why ctruncate, in contrast to the  
> old truncate, is not adding the ISYM/Y column to the mtz file anymore?  
> Should a pointer be added to the Sharp manual about this?
> 
> Cheers,
> 
> Florian
> 
> On Aug 24, 2010, at 5:53 PM, David Veesler wrote:
> 
>> Dear Florian,
>> to overcome this issue the simplest way is to just re-run your scala  
>> job but choosing the "old truncate".
>> That will generate the missing ISYM column.
>> 
>> All the best
>> David
>> 
>> Le 24 août 10 à 23:42, Florian Schmitzberger a écrit :
>> 
>>> Dear All,
>>> 
>>> The mtz file from my Scala run does not contain the ISYM/Y column,
>>> required for Autosharp. From the ccp4i gui I could not find an option
>>> to add the column within the Scala window to the mtz file.
>>> 
>>> I was wondering if one needs to run truncate separately, after scala,
>>> to add the ISYM column for the mtz file?
>>> 
>>> Thank you in advance!
>>> 
>>> Florian
>>> 
>>> -----------------------------------------------------------
>>> Florian Schmitzberger
>>> Biological Chemistry and Molecular Pharmacology
>>> Harvard Medical School
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>>> Tel: 001 617 432 5602
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> -----------------------------------------------------------
> Florian Schmitzberger
> Biological Chemistry and Molecular Pharmacology
> Harvard Medical School
> 250 Longwood Avenue, SGM 130
> Boston, MA 02115, US
> Tel: 001 617 432 5602
> 
> 
> 
> 
> 
> 
> 
> 
> 
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