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Sir,</SPAN><SPAN lang=EN-US
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size=3> <o:p></o:p></FONT></SPAN></P>
<P class=MsoNormal
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style="FONT-SIZE: 10pt; FONT-FAMILY: 新細明體; mso-bidi-font-family: 新細明體; mso-font-kerning: 0pt">I
have used autoSHARP in MAD mode. I got trouble in automatic building. The
autoSHARP set as follow: merged of three wavelength data, input heavy atom
sites (12 sites) and input protein sequence. When the autoSHARP proceed
automatic building, until 30 or 40 cycles the program is terminated. The error
show: PIR file sceond line must be blank! But input sequence is follow IF-3C.pir
and KrEl.pir I have no idea what is wrong with the input sequence. Would you
give me any recommend? </SPAN><SPAN lang=EN-US
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size=3> <o:p></o:p></FONT></SPAN></P>
<P class=MsoNormal
style="MARGIN: 0cm 0cm 0pt; mso-pagination: widow-orphan"><SPAN lang=EN-US
style="FONT-SIZE: 10pt; FONT-FAMILY: 新細明體; mso-bidi-font-family: 新細明體; mso-font-kerning: 0pt">The
addition file is the error message and the format of input sequence. Calculate
by the Matthews Coefficient that show probably 2 molecules so input sequence
contains 2 molecules.</SPAN><SPAN lang=EN-US
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<P class=MsoNormal
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size=3>Thanks in advanced.<o:p></o:p></FONT></SPAN></P>
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<P class=MsoNormal
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style="FONT-SIZE: 10pt; FONT-FAMILY: 新細明體; mso-bidi-font-family: 新細明體; mso-font-kerning: 0pt">cholien.</SPAN><SPAN
lang=EN-US
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