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<DIV><FONT face=Arial size=2>Hi, </FONT></DIV>
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<DIV><FONT face=Arial size=2>I have found Se sites using autoSharp with
resolution limits 30-6.0 angstroms (as suggested by NOTES within
autoSHARP). Yet my actual dataset (as well as native dataset) is up to 2.4
angstroms. What is the best approach to do phase extension to higher
resolution cutoff within SHARP/autoSHARP? </FONT></DIV>
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<DIV><FONT face=Arial size=2>Could I use the initial sites that I find from ha
search and then refine and phase using highest resolution cutoff of
2.4? </FONT></DIV>
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<DIV><FONT face=Arial size=2>Thanks for all your advice in advance!</FONT></DIV>
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<DIV><FONT face=Arial size=2>Cheers,</FONT></DIV>
<DIV><FONT face=Arial size=2>Nick</FONT></DIV>
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<DIV><FONT face=Arial
size=2>________________________________________</FONT></DIV>
<DIV> </DIV>
<DIV><FONT face=Arial size=2>Nicholas Noinaj<BR>University of Kentucky School of
Medicine<BR>Department of Molecular and Cellular Biochemistry<BR>Biomedical
Biological Sciences Research Building, Rm 236<BR>741 S. Limestone<BR>Lexington,
Ky 40536<BR>Lab: 859-323-8183<BR>Home: 859-228-0978<BR><A
href="mailto:noinaj@uky.edu">noinaj@uky.edu</A><BR><A
href="http://www.noinaj.com">www.noinaj.com</A></FONT></DIV>
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