Hi Clemens,<br><br>Thanks for responding!<br><br>Unfortunately, I wanted to use external phase info (HLs) to find HA sites. I am still pretty new to all of this, so please let me know if my logic is incorrect.<br><br>I am plagued with poor data. I have low resolution data (3.5 angstroms at peak wavelength with signal down to 4 angstroms). I have a possible MR soln. I can make an anomalous difference map and locate 3 peaks that align well with methionines in the MR model. Unfortunately, the peaks are in close proximity to each other (2 sites are 4 angstroms apart, the third is equidistant from the other 2 sites at a distance of 7 angstroms). I have tried inputting these initial HA sites, but they are not kept during refinement. I was hoping Hendrickson-Lattman coefficients would help.<br>
<br>Thanks,<br>Steph<br><br><br><br><div class="gmail_quote">On Feb 18, 2008 3:46 AM, Clemens Vonrhein <<a href="mailto:vonrhein@globalphasing.com">vonrhein@globalphasing.com</a>> wrote:<br><blockquote class="gmail_quote" style="border-left: 1px solid rgb(204, 204, 204); margin: 0pt 0pt 0pt 0.8ex; padding-left: 1ex;">
Hi Steph,<br><br>no - this can only be done in SHARP. But your autoSHARP run will<br>(hopefully) created a sensible starting point for running SHARP -<br>after all, SHARP is the central part of autoSHARP.<br><br>Or are you trying to use external phase information (in the form of<br>
HLs) to find heavy atom sites in the first place?<br><br>Cheers<br><br>Clemens<br><div><div></div><div class="Wj3C7c"><br>On Fri, Feb 15, 2008 at 12:51:51PM -0600, Stephanie Swanson wrote:<br>> Hi,<br>><br>> I am a fairly new user of SHARP. I am trying to figure out if there is any<br>
> way to input Hendrickson-Lattman coefficients into an autoSHARP run; or can<br>> this only be done in SAHRP?<br>><br>> Thanks,<br>> Steph<br><br></div></div>> _______________________________________________<br>
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