Acknowledgements

Software used by BUSTER:

The BUSTER suite calls out to a large number of other programs, taking advantage of an enormous amount of development by a wide variety of authors:

• BUSTER has its origins in a program written to explore the use of statistical methods, based on entropy and likelihood, in phase determination. This program was subsequently connected to the least-squares-based TNT refinement package by Dale Tronrud and Lynn Ten Eyck to produce a maximum-likelihood refinement program. Although it has evolved a great deal since, it still uses many parts of TNT, and its use requires a TNT licence.

• The CCP4 suite of programs is used throughout BUSTER.

• Quantum-mechanical energies, used in refine and grade, are by default calculated using Martin Field's fdynamo package, whose documentation may be found here.

• Warren DeLano's pymol (http://www.pymol.org/) is used in refine to draw pictures of ligand sites

• visualise-geometry-coot uses Paul Emsley's coot.

• grade uses mogul, distributed by CCDC, to query the Cambridge Structural Database, and libcheck from CCP4 for the initial steps in the generation of restraints from SMILES strings.

• grade_PDB_ligand uses OpenBABEL (see http://www.openbabel.org) for format conversion and atom typing.

• For hydrogenating proteins we use the reduce program from Molprobity:

MOLPROBITY: structure validation and all-atom contact analysis
for nucleic acids and their complexes, Ian W. Davis, Laura Weston Murray, 
Jane S. Richardson and David C. Richardson, 
Nucl. Acids Res. (2004) 32 (suppl 2): W615-W619.

Funding for BUSTER development:

The Global Phasing consortium provides the majority of the funding for the development of BUSTER.

We would also like to acknowledge the support of the following EU projects:

• VIZIER (LSHG-CT-2004-511960)
• SILVER (FP7-HEALTH-F3-2010-260644)