BusterCCplot

BUSTER reciprocal-space CC plot

• The reciprocal-space correlation coefficient plots produced by BUSTER are useful for diagnosing larger-scale problems with your crystal and your input model.
• The plot can reveal problems in the data collection/processing stage, such as: low resolution overloads, data anisotropy and ice ring contamination.
• It can also reveal inadequacies in the weighting of phase information from partial MR models in certain situations of severe incompleteness.

Seeing the reciprocal-space CC plot

• BUSTER writes a reciprocal-space CC plot for every small cycle of refinement in an mtv format that can be viewed by plotmtv.
• In general only the initial or last plot is of interest
• It used to be a chore to find these plots and launch plotmtv
• So from the February 2011 release we provide a utility script that can be run:

graph_autobuster_recipCC -d refine.dir

• here refine.dir is the results directory as specified by refine -d
• The script launches plotmtv so this must be able to run for this to work (needs X11).
• By default the last reciprocal-space CC plot found in the run is displayed. If you want to see the first then use the -first option.
• The other option is -ice where lines marking expected wavelengths for ice rings are marked on the graph. You may need to use plotmtv's zoom feature if ice is used.

Interpretation

• What does an ideal correlation plot look like? What can I find out about my data collection by discrepancies from the ideal?
• What does the correlation plot look like if there is a substantial portion of my model missing?
• What are the precise definitions of the quantities plotted on this graph?
• What behaviours do the curves on this graph describe?

• return to BUSTER Output Interpretation

Page by Oliver Smart and Tom Womack 07 Feb 2011. Any questions regarding our software or this wiki should be directed to buster-develop@globalphasing.com