BUSTER reciprocal-space CC plot

  • The reciprocal-space correlation coefficient plots produced by BUSTER are useful for diagnosing larger-scale problems with your crystal and your input model.
  • The plot can reveal problems in the data collection/processing stage, such as: low resolution overloads, data anisotropy and ice ring contamination.
  • It can also reveal inadequacies in the weighting of phase information from partial MR models in certain situations of severe incompleteness.

Seeing the reciprocal-space CC plot

  • BUSTER writes a reciprocal-space CC plot for every small cycle of refinement in an mtv format that can be viewed by plotmtv.
    • In general only the initial or last plot is of interest
    • It used to be a chore to find these plots and launch plotmtv
    • So from the February 2011 release we provide a utility script that can be run:
graph_autobuster_recipCC -d refine.dir
      • here refine.dir is the results directory as specified by refine -d
      • The script launches plotmtv so this must be able to run for this to work (needs X11).
      • By default the last reciprocal-space CC plot found in the run is displayed. If you want to see the first then use the -first option.
      • The other option is -ice where lines marking expected wavelengths for ice rings are marked on the graph. You may need to use plotmtv's zoom feature if ice is used.


Page by Oliver Smart and Tom Womack 07 Feb 2011. Any questions regarding our software or this wiki should be directed to buster-develop@globalphasing.com