RhofitFAQs

rhofit problems

• rhofit does not work because there is no difference density in the binding site: suggestions of how to run autoBUSTER to get better ligand difference density
• There is clear difference density for the ligand in my map, but rhofit tries to fit it somewhere incorrect. Try the -allclusters option
• There is clear difference density in my map, yet rhofit does not find a cluster in the binding site? See rhofitFAQ_manually_producing_cluster_files.
• rhofit has found the correct site to fit but the ligand has not been fitted correctly. See RhofitFAQbadFitAdvice

other FAQs

• I have a ligand that binds covalently: how should I use rhofit? See RhofitFAQcovalentLigand
• Can I run rhofit on an mtz file output by refmac? Yes!

Page by Oliver Smart,Tom Womack and Andrew Sharff. Original version 2 November 2009. Revised 9 April 2014. Address problems, corrections and clarifications to buster-develop@globalphasing.com