[buster-discuss] rhofit ??

[Oliver Smart]

On Wed, 29 Jun 2011, Jonathan Grimes wrote:

>
>   Dear Buster Developers,
>
>    We have solved the structure of a viral polymerase bound
>    to viral adaptor protein.   We can see clear density for a peptide
>    10-15aa, so much smaller than the full length adaptor
>    protein.   We dont know if the rest of the adaptor protein is in crystal (and
>    is disordered) or had been cleaved.
>    We are also unclear of the peptide sequence at the moment (data to ~2.3A).
>
>    Is ligand fitting Rhofit appropriate to use ??  Ive never used Rhofit but have
>   only heard good stories.
>
>    Thanks
>    Jon
>


Jon,

We have never tried rhofit on something this big. It handles cyclodextran 
but this sound like a case for normal coot type building?

regards,

Oliver

| Dr Oliver Smart                             |
| Global Phasing Ltd., Cambridge UK           |
| http://www.globalphasing.com/people/osmart/ |