[sharp-discuss] resid maps and final sharp round

[Clemens Vonrhein]

Dear Joseph,

Are you running the latest version of SUSHI (3.0.9) together with
SHARP 1.4.0? There were/are a few issues with this step. However, the
error you describe is new to me - I can't find any place where '+ }'
can occur. Could you send me the files check_input.asc and SIN for the
first SHARP run (the one that went through).

BTW: it is not too uncommon to have a few disordered Met residues -
and therefore miss some Se sites. Is it perhaps an N-terminal Met?

Hope that helps.

Cheers

Clemens

On Thu, Aug 08, 2002 at 08:44:21PM -0500, Joe Brunzelle wrote:
> I'm using autoSharp to determine a MAD structure (3 lambdas) which has 3 
> SeMet sites per molecule and the AU has 2 molecules.  The input file contains these 
> parameters for total AU.  During the RANTAN part 4 sites are found and 
> piped to sharp (sharp run completes). Then during the residual map 
> interpretation part only 3 of the 4 sites are detected in the maps.  This 
> is consistent for all 3 lambdas (steps 1-3).  When the routine goes to 
> step 4. Removing missing C-SITES the following error is reported:
> 
> syntax error at /progs2/sharp_new/bin/sharp/CsiteDel.pl line 88, near "+ }"
> Execution of /progs2/sharp_new/bin/sharp/CsiteDel.pl aborted due to compilation errors. 
> 
> Also, when you look at the new SIN file to be used in the final round of 
> Sharp, it is empty except for this
> ATOMS {
> }
> G-SITES {
> }
> END
> 
> Has anyone seen this before or are there any suggestions to correct for 
> this.
> 
> Thanks
> 
> -- 
> Joseph S. Brunzelle, Ph.D.
> Molecular Pharmacology and Biological Chemistry
> Northwestern University Medical School
> Ward Building, Room 8-264 Mail Code S215
> 303 E. Chicago Ave.
> Chicago, IL 60611-3008
> Phone (312) 503-0813
> FAX (312) 503-5349
> joeb@xtal.structure.northwestern.edu
> 
> 
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