[sharp-discuss] Native Fe in SHARP phasing

[Edward Berry]

A question about the SHARP output files eden*.mtz:
If I use native irons of my protein for phasing (anomalous and 
dispersive differences at different wavelengths) together with
regular heavy atom derivatives, does the iron contribution get
subtracted out before making the "native" protein mtz file F's 
(FPsha and FPshasol)? If I want to make a map with the iron 
density, should I use my native amplitudes together with 
PHIshasol and FOMshasol?

Thanks,
Ed