[sharp-discuss] Got trouble in autoSHARP-automatic building

[Claus Flensburg]

Dear Cholien,

On Mon, Nov 01, 2004 at 12:06:05PM +0800, ???? wrote:

> I have used autoSHARP in MAD mode. I got trouble in automatic
> building.  The autoSHARP set as follow: merged of three wavelength
> data, input heavy atom sites (12 sites) and input protein sequence.
> When the autoSHARP proceed automatic building, until 30 or 40 cycles
> the program is terminated. The error show: PIR file sceond line must
> be blank! But input sequence is follow IF-3C.pir and KrEl.pir I have
> no idea what is wrong with the input sequence. Would you give me any
> recommend?

I couldn't see your sequence file among the attachments. Please make
sure the ${name}.pir file has the following format (FASTA):

line 1: a greather than sign (>) followed by a text string (preferably
        the structure name)

line 2: nothing (a blank line)

line 3 and following lines : the one-letter sequence. Terminate with
an asterisk (*).

The width of line 3 and on-wards should be 60 or fewer characters.

Hope this helps,

ClAuS