[sharp-discuss] Hendrickson-Lattman coefficients

[Stephanie Swanson]

Hi Clemens,

Thanks for responding!

Unfortunately, I wanted to use external phase info (HLs) to find HA sites.
I am still pretty new to all of this, so please let me know if my logic is
incorrect.

I am plagued with poor data.  I have low resolution data (3.5 angstroms at
peak wavelength with signal down to 4 angstroms).  I have a possible MR
soln.  I can make an anomalous difference map and locate 3 peaks that align
well with methionines in the MR model.  Unfortunately, the peaks are in
close proximity to each other (2 sites are 4 angstroms apart, the third is
equidistant from the other 2 sites at a distance of 7 angstroms).  I have
tried inputting these initial HA sites, but they are not kept during
refinement.  I was hoping Hendrickson-Lattman coefficients would help.

Thanks,
Steph



On Feb 18, 2008 3:46 AM, Clemens Vonrhein <vonrhein at globalphasing.com>
wrote:

> Hi Steph,
>
> no - this can only be done in SHARP. But your autoSHARP run will
> (hopefully) created a sensible starting point for running SHARP -
> after all, SHARP is the central part of autoSHARP.
>
> Or are you trying to use external phase information (in the form of
> HLs) to find heavy atom sites in the first place?
>
> Cheers
>
> Clemens
>
> On Fri, Feb 15, 2008 at 12:51:51PM -0600, Stephanie Swanson wrote:
> > Hi,
> >
> > I am a fairly new user of SHARP.  I am trying to figure out if there is
> any
> > way to input Hendrickson-Lattman coefficients into an autoSHARP run; or
> can
> > this only be done in SAHRP?
> >
> > Thanks,
> > Steph
>
> > _______________________________________________
> > sharp-discuss mailing list
> > sharp-discuss at globalphasing.com
> > https://www.globalphasing.com/mailman/listinfo/sharp-discuss
>
>
> --
>
> ***************************************************************
> * Clemens Vonrhein, Ph.D.     vonrhein AT GlobalPhasing DOT com
> *
> *  Global Phasing Ltd.
> *  Sheraton House, Castle Park
> *  Cambridge CB3 0AX, UK
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