[sharp-discuss] Problem trying to use partial model as external phase information

Kevin chenqf at gmail.com
Thu Apr 23 01:47:06 CEST 2015

Hi all,

Recently I was able to get the density map of a protein at fairly low
resolution (~3.8A) using SIRAS in SHARP. Since it is a membrane protein and
the secondary structures are mostly transmembrane helices connected by
loops, I was able to build a rough partial poly ala model except some
broken loop regions. I wanted to try the external phase information feature
in SHARP and followed the manual to generate Hendrickson-Lattman
coefficients, however, I was not able to see my partial model in the drop
down list in "Phase Improvement and Interpretation Panel". I can see some
other pdb files but not the one I uploaded, which was in the datafiles
directory when I manually checked it. Does the pdb file need some special
headers in order to be visible in the list?

Any help will be appreciated.

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