[sharp-discuss] Problem trying to use partial model as external phase information

[Gupta, Yogesh]

Qingfeng,

Just add "model_"  to your pdb file name. For example, if your file name is test.pdb, change its name to
model_test.pdb.

Yogesh

Yogesh Gupta, PhD
Assistant Professor
Dept. of Structural and Chemical Biology
Mount Sinai School of Medicine
1425 Madison Ave, Icahn/East Bldg. Room # 16-23B
New York, NY 10029
Tel: +1-212-659-8713
http://www.mountsinai.org/profiles/yogesh-gupta
________________________________
From: sharp-discuss [sharp-discuss-bounces at globalphasing.com] on behalf of Kevin [chenqf at gmail.com]
Sent: Wednesday, April 22, 2015 7:47 PM
To: sharp-discuss at globalphasing.com
Subject: [sharp-discuss] Problem trying to use partial model as external phase information

Hi all,

Recently I was able to get the density map of a protein at fairly low resolution (~3.8A) using SIRAS in SHARP. Since it is a membrane protein and the secondary structures are mostly transmembrane helices connected by loops, I was able to build a rough partial poly ala model except some broken loop regions. I wanted to try the external phase information feature in SHARP and followed the manual to generate Hendrickson-Lattman coefficients, however, I was not able to see my partial model in the drop down list in "Phase Improvement and Interpretation Panel". I can see some other pdb files but not the one I uploaded, which was in the datafiles directory when I manually checked it. Does the pdb file need some special headers in order to be visible in the list?

Any help will be appreciated.

Qingfeng
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